Alternatively, the singlet excited state can undergo inter-system crossing to the triplet state, which will relax back to the singlet ground-state typically on a millisecond timescale Ref 2. This state is typically short-lived (a few nanoseconds), and rapidly decays back to the ground state, either through the emission of a photon (fluorescence), or by non-radiative relaxation. a fluorescent protein) in the electronic ground state absorbs a photon of suitable wavelength, it accesses the first electronically excited state. As shown on the figure (so called Jablonski diagram), when a molecule (e.g. Our dayly bread : Understanding the molecular mechanismsįP Function : A dog walking on his hind legs ?įluorescence is a process by which an electronically excited molecule or atom spontaneously emits a photon in order to relax to its ground state (Ref 1). Smart labels : Photo-Transformable Fluorescent Proteins (PTFPs) Key parameters : Brightness, Colour, Monomericity, pKa The Chromophore : structure & spectral propertiesĮxtending the palette : Fishing & Engineering The Green Fluorescent Protein (GFP) : Discovery & Structure
Meos3.2 photoconvert pdf#
This section is largely based on the introduction chapter of Daniel Thedié’s PhD thesis (tel-02613823v1) with input from Virgile Adam, Romain Berardozzi, Dominique Bourgeois, Martin Byrdin, Oleksandr Glushonkov, Angela Mantovanelli, Lukas Rane and Jip Wulffele (spring 2021)Ī pdf version of this document can be downloaded here.
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